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ZDB-FIG-110610-2

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Wilkinson et al., 2009 - Hedgehog and Bmp polarize hematopoietic stem cell emergence in the zebrafish dorsal aorta

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Fig. S2

Purmorphamine Treatment Does Not Affect Initiation or Maintenance of gfi1 Expression Within the Zebrafish DA

The initiation of expression of an additional marker of HSCs (Hock et al., 2004) (Zeng et al., 2004), gfi1 (Dufourcq et al., 2004), is unaffected following elevation of Hh. It is of note that, on average, 66% of wild-type zebrafish embryos express gfi1 within the DA at any time between 23 and 30hpf, which suggests that its expression within the HSCs is cycling (data not shown). a-h) Initiation and maintenance of gfi1 in the DA (red arrowheads) is unaffected in embryos treated with purmorphamine from mid blastula transition (b, d, f, h) in comparison to DMSO treated controls (a, c, e, g). Since a percentage of embryos fail to express gfi1 at each time point (data not shown), indicating that gfi1 expression is highly dynamic and may be cycling within the DA, embryo numbers relate to the number of embryos expressing gfi1 within the DA and not the number of positive cells.

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Cell, 16(6), Wilkinson, R.N., Pouget, C., Gering, M., Russell, A.J., Davies, S.G., Kimelman, D., and Patient, R., Hedgehog and Bmp polarize hematopoietic stem cell emergence in the zebrafish dorsal aorta, 909-916, Copyright (2009) with permission from Elsevier. Full text @ Dev. Cell