Fig. S6

Expression of gfi1 Within the DA is Lost in a Similar Manner to runx1 Following Bmp Inhibition

a-h) Expression of gfi1 (red arrowheads) in embryos heat shocked at 21hpf. Whereas gfi1 is expressed from 23-26hpf in two thirds of control GFP- (black letters) embryos, gfi1 is lost from the DA in 75% of GFP+ (green letters) embryos at 23hpf and 89% of GFP+ embryos at 24hpf, in a similar pattern to that observed for runx1 following induction of tBR at the same stages. i, j) Whilst gfi1 is expressed within the DA at 26hpf (red arrowheads) in 75% of uninjected controls (i), expression of gfi1 is absent from the DA in 77% of embryos injected with bmp4 morpholino (j). Taken together, these results indicate that temporal inhibition of Bmp signalling (a-h) results in temporal inhibition of gfi1 followed by recovery, but that permanent inhibition of Bmp via bmp4 morpholino injection results in a more permanent inhibition of gfi1 expression within the DA in a similar manner to that observed for runx1. Embryos were over stained to ensure that lack of gfi1 expression in the DA was not an artefact of reduced staining.