ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

VDD in male 6 mpf zebrafish fed a vitamin D null diet. (A) VDD fish had lower whole body levels of both 25(OH)D3 and 1,25(OH)2D3 than lab diet and VD3 sufficient fish. The limit of quanititation (LOQ) was 8 pmol/g and the limit of detection (LOD) was 3 pmol/g. Data are represented as mean ± SD. (B) VDD livers expressed significantly more cyp27b1 and cyp2r1, and significantly less cy24a1, than vitamin D sufficient livers. Data are represented as mean ± SE. *See also Supplementary Table 2.

Stunted growth observed in the VDD zebrafish 6 mpf. (A) Both male and female VDD zebrafish exhibited stunted growth 6 mpf. Descending order: MALE (lab diet, VD3 sufficient, VDD), FEMALE (lab diet, VD3 sufficient, VDD). (B) Growth rate was taken biweekly starting at 2 mpf and ending at 6 mpf (2 mpf, 2.5 mpf, 3 mpf, etc.). At 6 mpf, the average SL (cm) for VDD, VD3 sufficient, and LD fish was 1.33 ± 0.0 cm, 2.21 ± 0.06 cm, and 2.25 ± -0.06 cm, respectively. Data are represented as mean ± SEM. (C) The SGR (%) from 2–6 mpf for VDD, VD3 sufficient, and LD fish was 1.72%, 2.69%, and 2.93%, respectively. Data are represented as mean ± SEM. All measures are representative of a mixed gender population. *See also Supplementary Figs. 1 and 2.

Metabolic homeostasis in VDD liver. (A) VDD fish had significantly elevated levels of hepatic triglycerides (662.37 ± 46.25 mg/dL) 6 mpf. Data are represented as mean ± SEM. (B–D) VDD fish had lower plasma FFA levels (19.67 ± 5.13uM), lower total, HDL, LDL/VLDL, and free hepatic cholesterol (822.7 ± 42.49 mg/dL, 884.9 ± 49.50 mg/dL, 52.7 ± 8.74 mg/dL, 956.2 ± 96.39 mg/dL, respectively) and lower plasma LPL activity (18.5 nmol/min/mL) 6 mpf. Data are represented as mean ± SEM.

VDD fish demonstrated both hypertrophy and hyperplasia of dorsal paraosseal (dPOS) non-visceral AT (NVAT) and pancreatic visceral AT (PVAT)³¹. (A) Representative images of dPOS NVAT (58.08 mm² area). (B) Representative images of PVAT (58.08 mm² area). (C) VDD fish had hypertrophic and hyperplastic (60.78 ± 9.09 adipocytes) dPOS NVAT compared to VD3 sufficient 6 mpf. Data are represented as mean ± SEM (n = 9). (D) VDD fish had hypertrophic and hyperplastic (64.75 ± 15.11 adipocytes) PVAT compared to VD3 sufficient 6 mpf. Data are represented as mean ± SEM (n = 9).

Metabolic dyshomeostasis in VDD AT. (A) Elevated expression of lipid transporters in VDD liver 6 mpf. Decreased expression of fabp11a in VDD AT 6 mpf. (B) Elevated expression of lypolitic factors in VDD liver 6 mpf, with a decrease in lipea and lipeb in VDD AT. (C) Greater abundance of erk1/2 protein in VDD liver 6 mpf, with a decreased in p-erk1/2. (D) Suppression of mitochondrial biogenesis in VDD AT compared to VDD liver 6 mpf. (E) Elevated expression of lipogenic factors in VDD liver 6 mpf. Elevated expression of pparg and srebf1 in VDD AT 6 mpf with a decrease in pparaa and cebpa. *See also Figure S3.

Evaluating GH signaling in VDD liver, AT, and brain. (A,B) VDD fish had significantly elevated expression of ghr in both liver and brain 6 mpf, but no difference in gh1. (C,D) VDD fish had significantly elevated expression of igf in liver 6 mpf, with only a slight increase in igf1 in the AT. (E) No difference seen in GH protein abundance in the brain.

Evaluating INS signaling in VDD liver and AT. (A,B) VDD fish had significantly elevated expression of insig1, insig2, with a significant decrease in cidec in liver 6 mpf, indicating sustained INS signaling contrary to also having significantly elevated expression of cish, socs2, and socs3b, suppressors of cytokine signaling thought to shut down the INS signaling cascade. (C) Downregulation of insig1 and cidec in VDD AT 6 mpf.

Summarizing the impact of VDD. VDD during early life stage development leads to metabolic dyshomeostasis, where there is an imbalance between somatic growth and adiposity.