Fig. 2

Loss of Crhr1 abolish the thermal stressor-induced hyperactivity: The top panel shows the total distance moved in the light by the wildtype (WT) and crhr1−/− larvae (KO) in response to 28 °C (28) or 33 °C (33) at 15 (A, only main effect, P = 0.004) or 60 min (B, significant interaction and no main effect, P = 0.0002). Boxes represent 1 SD above and below the mean of the data (n = 84–188, 4–8 trials with 24 larvae each), the line inside the box shows the median of the data, and the whiskers indicate the minimum and maximum of all the data; boxes with different letters are significantly different (nonparametric Two-way ANOVA). The bottom panel shows the total distance moved by the wildtype without (WT) and with antalarmin (WT + An) in response to 28 °C (28) or 33 °C (33) at 15 (C, only interaction, asterisk (∗) denotes significant difference between two group, P = 0.02) or 60 min (D, significant interaction but no main effect, P = 0.00003). Boxes represent 1 SD above and below the mean of the data (n = 34–90, 2–4 trials with 24 larvae each), the line inside the box shows the median of the data, and the whiskers indicate the minimum and maximum of all the data; The main effect is shown as an inset (Fig. 2A), while different letter or asterisk (∗) denotes significant interactions (between groups); boxes with different letters are significantly different (nonparametric Two-way ANOVA).