FIGURE

Figure 7.

ID

ZDB-FIG-250320-13

Publication

Doan et al., 2025 - Dock1 functions in Schwann cells to regulate development, maintenance, and repair

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Figure 7.

Targeted genetic approaches reveal an interaction between Dock1 and Rac1 in the developing PNS. (A–I) TEM micrographs of cross-sections of the PLLn, showing myelinated axons pseudocolored in blue, in control (Cas9 only), sgRac1+Cas9, and sgRac3+Cas9 in WT dock1^+/+, HET dock1^stl145/+, and homozygous dock1^{stl145/stl145} MUT zebrafish. (J) Quantification of myelinated axons in the PLLn. n = 6 fish per genotype and experimental condition. (K) Western blot showing sciatic nerve active and total Rac1 protein levels from control and Dock1 cKO animals, along with quantification of protein levels. Representative blot consisting of 12 nerves from 6 animals per genotype. Here, and in all figures, the X symbol in the graph denotes a data point corresponding to the representative image shown. (A–I) Scale bar = 100 µm. (J) Two-way ANOVA with Sidak’s multiple comparisons test. ***P < 0.001; *P < 0.05; ****P < 0.0001; ns, not significant. Source data are available for this figure: SourceData F7.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dock1^{stl145/stl145} WT + CRISPR1-rac3a WT + CRISPR2-rac1a dock1^stl145/+ dock1^stl145/+ + CRISPR1-rac3a dock1^stl145/+ + CRISPR2-rac1a dock1^{stl145/stl145} + CRISPR1-rac3a dock1^{stl145/stl145} + CRISPR2-rac1a
Knockdown Reagents:	CRISPR1-rac3a CRISPR2-rac1a
Observed In:	posterior lateral line myelin sheath
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.