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NELO and its cohesion with ILTs and ALTs in other teleost fish species. (A and B) Cryosection from wild crucian carp labeled with anti-ZAP70 antibody (magenta hot). This larger representative of the cyprinids than zebrafish also has NELO (red arrowheads), ILTs (yellow stars), and ALTs (cyan stars), which are all interconnected by a clear lymphoid network. (C) Zoom from (B) in which phalloidin (orange hot) and DAPI (cyan hot) staining revealed the presence of endothelial vessels containing red blood cells. Transversal (D) and coronal (E) cryosections of an adult Atlantic salmon (1.5 kg) labeled with anti-ZAP70 (magenta hot) displaying NELO (red arrowheads), ILTs (yellow stars), and ALTs (cyan stars). As for cyprinids, NELO (red arrowheads) of this representative of the salmonid family is located at the ventral convergence of gill arches, interconnected with gills lymphoid aggregates, and closely associated with the urohyal bone. It is particularly enriched in ZAP70-positive cells (F). In contrast, the mucosal epithelium lining the region directly anterior to the urohyal bone only displayed scarce T/NK cell (G). Annotations: B, bone; Ec, endothelial cell; Lu, lumen; Rbc, red blood cell. Scale bars, 1000 (A), 500 (D), 400 (E), 100 (B), 50 (F and G), and 20 μm (C).
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