PUBLICATION
Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?
- Authors
- Resseguier, J., Nguyen-Chi, M., Wohlmann, J., Rigaudeau, D., Salinas, I., Oehlers, S.H., Wiegertjes, G.F., Johansen, F.E., Qiao, S.W., Koppang, E.O., Verrier, B., Boudinot, P., Griffiths, G.
- ID
- ZDB-PUB-231102-4
- Date
- 2023
- Source
- Science advances 9: eadj0101 (Journal)
- Registered Authors
- Nguyen-Chi, Mai, Oehlers, Stefan, Resseguier, Julien
- Keywords
- none
- MeSH Terms
-
- Humans
- Mammals
- Zebrafish*
- Lymphoid Tissue
- Pharynx
- Animals
- T-Lymphocytes
- Palatine Tonsil*
- PubMed
- 37910624 Full text @ Sci Adv
Citation
Resseguier, J., Nguyen-Chi, M., Wohlmann, J., Rigaudeau, D., Salinas, I., Oehlers, S.H., Wiegertjes, G.F., Johansen, F.E., Qiao, S.W., Koppang, E.O., Verrier, B., Boudinot, P., Griffiths, G. (2023) Identification of a pharyngeal mucosal lymphoid organ in zebrafish and other teleosts: Tonsils in fish?. Science advances. 9:eadj0101.
Abstract
The constant exposure of the fish branchial cavity to aquatic pathogens causes local mucosal immune responses to be extremely important for their survival. Here, we used a marker for T lymphocytes/natural killer (NK) cells (ZAP70) and advanced imaging techniques to investigate the lymphoid architecture of the zebrafish branchial cavity. We identified a sub-pharyngeal lymphoid organ, which we tentatively named "Nemausean lymphoid organ" (NELO). NELO is enriched in T/NK cells, plasma/B cells, and antigen-presenting cells embedded in a network of reticulated epithelial cells. The presence of activated T cells and lymphocyte proliferation, but not V(D)J recombination or hematopoiesis, suggests that NELO is a secondary lymphoid organ. In response to infection, NELO displays structural changes including the formation of T/NK cell clusters. NELO and gill lymphoid tissues form a cohesive unit within a large mucosal lymphoid network. Collectively, we reveal an unreported mucosal lymphoid organ reminiscent of mammalian tonsils that evolved in multiple teleost fish families.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping