Figure 5

Gpr126 is expressed throughout the urinary collecting system. (a) RNAscope^® probes targeting the human GPR126 mRNA were used on human FFPE urinary tissues. GPR126 signal (magenta dots) was found in the epithelial compartment of the urinary collecting system, collecting duct epithelial cells and transitional epithelium (urothelium) of the renal pelvis, ureter, and bladder. Hematoxylin was utilized to visualize nuclei. Scale bars: 20 μm. (b) RNAscope^® probes targeting Gpr126 mRNAs were used on FFPE renal pelvis sections of human and mouse. Both species showed Gpr126 enrichment in the urothelium (U) of the renal pelvis mucosa (yellow dots), contrary to the lamina propria (LP) layer beneath. DAPI was utilized to visualize nuclei. Dashed red line represents the approximate location of the basement membrane at the urothelium–lamina propria junction. Scale bars: 50 μm.