Figure 5

Exposure to DomA at 2 dpf does not grossly alter selected sensory neuron structures or the main axons of two of the three primary motor neurons. (A) Diagram of 2.5 dpf embryos laterally mounted and immunostained with anti-acetylated tubulin. The rectangle delineates the approximate area that was imaged. (B) Representative images of control (n = 35) and DomA-exposed larvae (n = 29) immunostained with anti-acetylated tubulin. Labels point to both the sensory ganglia that comprise the anterior lateral line (nADso, nVDI, nADb, nAVm) and to the peripheral lateral line (pLL). (C) Diagram of the axonal trajectories in the three types of primary motor neurons assessed in Tg(olig2:EGFP) fish. (D) Representative images of Tg(olig2:EGFP) × Tg(sox10:mRFP) fish exposed to vehicle or to DomA at 2 dpf then imaged at 2.5 dpf. Peach arrows point to the MiP main axons, magenta arrows point to the RoP main axons, and teal arrows point to the CaP main axons. Scale bar = 50 μm. These fish were the same fish used in Fig. 1, with only the EGFP shown. (E) Presence or absence of intact main axons of the three types of primary motor neurons in control and DomA-exposed fish. ***p < 0.001. Abbreviations: nADso superior opthalmic ramus of the anterodorsal lateral line nerve, nVDI dorsolateral nerve of the trigeminal ganglion, nADb buccal ramus of the anterodorsal lateral line nerve or nAVm the mandibular ramus of the anterior lateral line nerve. MiP Middle primary motor neuron, RoP rostral primary motor neuron, CaP Caudal primary motor neurons.