Fig. 3
optoSynC activation reduces miniature post-synaptic current (mPSC) rate at the neuromuscular junction (NMJ) and can block cholinergic transmission for hours.
a Representative postsynaptic current traces recorded in body wall muscle cells of optoSynC-expressing animals; blue light pulses (470 nm, 8 mW/mm², 5 s / 5 s ISI) indicated by blue rectangles. b Close-up of regions indicated in a, before (0–10 s, dark blue) and after (95–105 s, light blue) blue light illumination. c Mean (±s.e.m.) mPSC frequency with blue light illumination of wild type and optoSynC expressing animals. d Group analysis of data (mean ± s.e.m.) in c, intervals before (0–30 s) and after (76–105 s) illumination. Number of independent animals is n = 8 (wild type) and n = 18 (optoSynC). e, f Analysis of mPSC amplitude (mean ± s.e.m.), as in c, d. g Paralysis of animals in response to 2 (or 0) mM aldicarb under continuous blue light illumination (470 nm, 0.05 mW/mm²), or in the dark, as indicated (mean ± s.e.m.). Two-way ANOVA with Bonferroni correction of the indicated number of animals (in b–f), or of N = 3 experiments averaged at the indicated time points with n independent animals as indicated (in g). ***p < 0.001, ns not significant. |