ZFIN Image: Vettkötter et al., 2022, Fig. 3

Image

Figure Caption

Fig. 3

optoSynC activation reduces miniature post-synaptic current (mPSC) rate at the neuromuscular junction (NMJ) and can block cholinergic transmission for hours.

a Representative postsynaptic current traces recorded in body wall muscle cells of optoSynC-expressing animals; blue light pulses (470 nm, 8 mW/mm², 5 s / 5 s ISI) indicated by blue rectangles. b Close-up of regions indicated in a, before (0–10 s, dark blue) and after (95–105 s, light blue) blue light illumination. c Mean (±s.e.m.) mPSC frequency with blue light illumination of wild type and optoSynC expressing animals. d Group analysis of data (mean ± s.e.m.) in c, intervals before (0–30 s) and after (76–105 s) illumination. Number of independent animals is n = 8 (wild type) and n = 18 (optoSynC). e, f Analysis of mPSC amplitude (mean ± s.e.m.), as in c, d. g Paralysis of animals in response to 2 (or 0) mM aldicarb under continuous blue light illumination (470 nm, 0.05 mW/mm²), or in the dark, as indicated (mean ± s.e.m.). Two-way ANOVA with Bonferroni correction of the indicated number of animals (in b–f), or of N = 3 experiments averaged at the indicated time points with n independent animals as indicated (in g). ***p < 0.001, ns not significant.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.