FIGURE

Fig. 5

ID

ZDB-FIG-220517-47

Publication

Macklin et al., 2022 - Intrinsic epigenetic control of angiogenesis in induced pluripotent stem cell-derived endothelium regulates vascular regeneration

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Fig. 5

hiPSC-EC VEGFR2 expression is regulated by HAT P300 activity.

RT-qPCR for VEGFR2 in C1-2 iECs treated with a TSA, b CPTH6, and c C646. (N = 3, n = 9). d Schematic is outlining of P300 HAT VEGFR2 regulation and C646 inhibition in iECs. Schematic created with BioRender.com. e Flow cytometry for VEGFR2 on iECs treated with C646 for varying periods (C-Control, ECGM; VC-Vehicle Control, DMSO; 1,3,18, and 24 h) N = 3. f ChIP qPCR data are shown as binding events per 1000 cells to the +57 K location at the VEGFR2 transcriptional start site. N = 2. g Representative confocal microscopy images of C1-2 iECs spheroids cultured in media supplemented with VEGF or VEGF and C646. (N = 2, n = 10) (N = 2, n = 10). Statistical significance levels are set at *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001 and Tukey’s multiple comparison test by two-tailed Student’s t-test and Tukey’s multiple comparison test. Data are presented as mean ± SD. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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