FIGURE

Figure 4

ID

ZDB-FIG-211029-101

Publication

D'Gama et al., 2021 - Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain

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Figure 4

gmnc is required for multiciliation in ependymal cells

(A and B) Absence of MCCs in 1-month-old (A1 and A2; n = 3 ctrl; 3 mutants) and adult brains of gmnc mutants (B1–B3; n = 3 ctrl; 3 mutants). Arrowheads show MCC in controls. All ciliated cells in gmnc mutant are monociliated (arrow). (A1) 1-month dorsal telencephalon and (A2) ChP. (B1) Adult dorsal telencephalon. (B2) Anterior portion of the adult ChP. (B3) Adult midline above the Ca.

(C1–C3) gmnc enhances the expression of foxj1a and foxj1b in the TC and ChP. (C1) HCR revealed a gmnc-dependent foxj1a expression, particularly in the anterior ChP (arrow in insets). n = 4 controls; 6 mutants. The hashtag symbol shows nonspecific signal in blood vessels. (C2) foxj1b:GFP remained expressed in gmnc mutant with reduced and more homogeneous levels (n = 4 controls; 7 mutants). β-catenin and glutamylated tubulin staining was used for quantification of GFP levels in C3. (C3) Monociliated cells express less foxj1b:GFP than MCCs and monociliated cells in gmnc mutant express similar foxj1b:GFP levels as control monociliated cells. All data points are plotted; mean and SD of n fish; ^∗p < 0.05 calculated on the average fluorescence per fish using rank sum test.

(D) HCR shows that foxj1a and foxj1b remained expressed in the telencephalic midline of gmnc mutant (n = 3).

Asterisk indicates axons. A, anterior; P, posterior; R, right; L, left; Tel, telencephalon.

See also Figure S3.

Expression Data

Genes:	EGFP foxj1a foxj1b gmnc
Fish:	WT gmnc^sq34/sq34 foxj1b^tsu10Gt gmnc^sq34/sq34; foxj1b^tsu10Gt
Anatomical Terms:	choroid plexus anterior region telencephalon telencephalon medial region
Stage Range:	Days 21-29 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gmnc^sq34/sq34 gmnc^sq34/sq34; foxj1b^tsu10Gt
Observed In:	choroid plexus choroid plexus anterior region dorsal telencephalon multi-ciliated epithelial cell telencephalon
Stage Range:	Days 21-29 to Adult

Phenotype Detail

Acknowledgments

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