FIGURE

Fig. 4

ID

ZDB-FIG-210501-14

Publication

Blokzijl-Franke et al., 2021 - Phosphatidylinositol-3 kinase signaling controls survival and stemness of hematopoietic stem and progenitor cells

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Fig. 4

HSPCs of LY294002-treated embryos engage in all blood lineages, but show impaired colonization of definitive hematopoietic organs.

a–h Control and LY294002-treated (from 32–60 hpf) embryos were fixed at 4 dpf. Markers for definitive blood lineages were assessed by in situ hybridization in the CHT: c-myb (HSPCs; a, b), globin (erythrocyte lineage; c, d), ikaros (lymphocyte lineage; e, f), l-plastin (leukocytes; g, h). Representative embryos are shown, with anterior to the left. The number of embryos that showed a particular pattern/total number of embryos is indicated in the bottom right corner of each panel. i, j GFP^high thrombocytes were imaged at 5 dpf in tg(cd41:eGFP) embryos. Scale bar: 100 µm. k–n High-resolution imaging at 12 dpf of kidney (k, l, dorsal view) (control, n = 6; LY294002-treated, n = 8; 4 µm step size) and thymus (m, n, lateral view) (control, n = 6; LY294002-treated, n = 7; 2 µm step size). Anterior to the left; maximum projections of representative larvae. Scale bar: 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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