FIGURE

Fig. 2

ID

ZDB-FIG-210210-3

Publication

Cambier et al., 2020 - Spreading of a mycobacterial cell surface lipid into host epithelial membranes promotes infectivity

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Fig. 2

Synthesis and application of a chemically tractable, biologically active PDIM variant, azido-DIM. (A) Synthesis of azido-DIM. (B) Model of delipidation and recoating of bacteria with or without azido-DIM followed by treatment with an azide-reactive cyclooctyne, DIBO-488. (C) Flow cytometry analysis of M. marinum recoated with or without azido-DIM treated with DIBO-488. Image of (D) native lipid control or (E) azido-DIM recoated bacteria treated with DIBO-488, scale bar = 8 μm. (F) Mean bacterial volume 3 days following HBV infection of wildtype fish with ~100 delipidated M. marinum recoated with Native, DIM-depleted (DIM–), DIM– plus native DIMs (+DIMs), or DIM– plus azido-DIM (+Azido-DIM) lipids. Kruskal-Wallis ANOVA for unequal variances with Dunn’s multiple comparisons test with selected adjusted P values shown. (C), (F) representative of three separate experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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