Figure 5—figure supplement 1.

Representative fluorescence recovery of Wnt3EGFP at a distal target site after photobleaching.

(A) Expression of Wnt3EGFP in Tg(−4.0wnt3:Wnt3EGFP) 48 hpf before photobleaching. (B) Photobleached region of Wnt3EGFP. (C) Recovery of fluorescence intensity in the bleached region due to diffusion of molecules from the neighboring unbleached regions. (D) Fluorescence recovery curve for Wnt3EGFP with a time constant (τfast) of ~6.5 min and an apparent global diffusion coefficient (Dglobal) of 0.47 μm2/s. Scale bar 30 μm. 

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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