Figure 5—figure supplement 2.

Representative fluorescence recovery of Wnt3EGFP at a distal target site after heparan sulfate proteoglycan (HSPG) disruption.

(A) Expression of Wnt3EGFP in Tg(−4.0wnt3:Wnt3EGFP) before photobleaching in HSPG disrupted embryos. (B) Photobleached region of Wnt3EGFP. (C) Recovery of fluorescence intensity in the bleached region due to diffusion from the neighboring unbleached regions. (D) Fluorescence recovery curve for Wnt3EGFP with a recovery time (τfast) of ~1.5–2.5 min and a Dglobal of ~1.5–3.75 μm2/s, which is approximately two to three times faster than untreated embryos as shown in Figure 5. Images orientation: anterior to the left. Scale bar 30 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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