Figure 2—figure supplement 1.

Fluorescence correlation spectroscopy (FCS) measurements of Wnt3EGFP and PMTmApple in the Wnt3 target regions at 24 hpf.

Expression of (A) PMTmApple and (B) Wnt3EGFP in Tg(−4.0wnt3:Wnt3EGFP) × Tg(−4.0wnt3:PMTmApple) double transgenic embryos at 24 hpf. Representative autocorrelation function (ACF) for (C) PMTmApple and (D) Wnt3EGFP in the OT at 24 hpf. No ACFs were obtained for PMTmAPple in the target regions indicating an absence of mApple signal in these regions. Ce, cerebellum; MHB; midbrain–hindbrain boundary; OT, optic tectum. Images orientation: anterior to the left. Scale bar 30 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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