Figure 5—figure supplement 3.

Representative fluorescence recovery of secEGFP after photobleaching at 48 hpf.

(A) Expression of secEGFP before photobleaching. (B) Photobleached region of secEGFP. (C) Recovery of fluorescence intensity in the bleached region due to diffusion of molecules from the neighboring unbleached regions. (D) Fluorescence recovery curve for secEGFP with a quick recovery time (τfast) of ~45 s and a fraction of mobile component (Fm) of ~0.85. The average global diffusion coefficient (Dglobal) measured for secEGFP was 13 ± 4 μm2/s. Images orientation: anterior to the left. Scale bar 30 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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