ZFIN ID: ZDB-FIG-201003-68
Rima et al., 2020 - Dynamic regulation of the cholinergic system in the spinal central nervous system. Scientific Reports   10(1):15338 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Figure 2

Spatial expression pattern of cholinergic genes in spinal inter- and motoneurons at 24 hpf. (A) Lateral view (left) and cross sections (right) of embryos processed by in situ hybridization showing hacta, chrna3, ache, and chrna7 transcripts in the spinal cord. While hacta, ache, and chrna7 transcripts are expressed in the ventral spinal cord (black arrowheads) that contains motoneurons (MNs), chrna3 is expressed in intermediate spinal cord (red arrowheads) consistent with location of interneurons (INs). The spinal cord is outlined by dashed lines. Scale bar: 30 µm. (B) Representative confocal images of fluorescent in situ hybridization of 24 hpf Tg(mnx1:GFP) embryos showing MNs in green (GFP) and hacta (first row), chrna3 (second row), ache (third row), and chrna7 (fourth row) transcript in magenta (white arrows). Scale bar: 15 µm. (C) Classification of hacta, chrna3, ache, and chrna7 expression in MNs (green) or non-MNs (grey). (D) Percentage of hacta, chrna3, ache, and chrna7 expressing mnx1:GFP neurons. hacta: N = 5 embryos (176 mnx1:GFP+ neurons, 171 hacta+ neurons). chrna3: N = 4 embryos (214 mnx1:GFP+ neurons, 77 chrna3+ neurons). ache: N = 5 (132 mnx1:GFP+ neurons, 128 ache+ neurons). chrna7: N = 8 embryos (223 mnx1:GFP+ neurons, 59 chrna7+ neurons).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ache AB standard conditions Prim-5 spinal cord ventral region ISH
ml2Tg standard conditions Prim-5 spinal cord motor neuron ISH
chrna3 AB standard conditions Prim-5 spinal cord medial region ISH
ml2Tg standard conditions Prim-5 spinal cord interneuron ISH
chrna7a AB standard conditions Prim-5 spinal cord ventral region ISH
ml2Tg standard conditions Prim-5 spinal cord motor neuron ISH
GFP ml2Tg standard conditions Prim-5 spinal cord motor neuron IHC
slc5a7a AB standard conditions Prim-5 spinal cord ventral region ISH
ml2Tg standard conditions Prim-5 spinal cord motor neuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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