PUBLICATION

Dynamic regulation of the cholinergic system in the spinal central nervous system

Authors
Rima, M., Lattouf, Y, Abi Younes, M., Bullier, E., Legendre, P., Mangin, J.M., Hong, E.
ID
ZDB-PUB-200924-1
Date
2020
Source
Scientific Reports   10(1): 15338 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Central Nervous System/embryology*
  • Central Nervous System/metabolism
  • Choline O-Acetyltransferase/genetics
  • Choline O-Acetyltransferase/metabolism
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental*
  • Larva/metabolism
  • Motor Neurons/metabolism
  • Neurons/physiology
  • Neurotransmitter Agents/metabolism
  • Spinal Cord/embryology*
  • Spinal Cord/metabolism
  • Vesicular Acetylcholine Transport Proteins/genetics
  • Vesicular Acetylcholine Transport Proteins/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
32948826 Full text @ Sci. Rep.
Abstract
While the role of cholinergic neurotransmission from motoneurons is well established during neuromuscular development, whether it regulates central nervous system development in the spinal cord is unclear. Zebrafish presents a powerful model to investigate how the cholinergic system is set up and evolves during neural circuit formation. In this study, we carried out a detailed spatiotemporal analysis of the cholinergic system in embryonic and larval zebrafish. In 1-day-old embryos, we show that spinal motoneurons express presynaptic cholinergic genes including choline acetyltransferase (chata), vesicular acetylcholine transporters (vachta, vachtb), high-affinity choline transporter (hacta) and acetylcholinesterase (ache), while nicotinic acetylcholine receptor (nAChR) subunits are mainly expressed in interneurons. However, in 3-day-old embryos, we found an unexpected decrease in presynaptic cholinergic transcript expression in a rostral to caudal gradient in the spinal cord, which continued during development. On the contrary, nAChR subunits remained highly expressed throughout the spinal cord. We found that protein and enzymatic activities of presynaptic cholinergic genes were also reduced in the rostral spinal cord. Our work demonstrating that cholinergic genes are initially expressed in the embryonic spinal cord, which is dynamically downregulated during development suggests that cholinergic signaling may play a pivotal role during the formation of intra-spinal locomotor circuit.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes
This article is corrected by ZDB-PUB-220906-268.