ZFIN ID: ZDB-FIG-200622-21
Müller et al., 2020 - RNA exosome mutations in pontocerebellar hypoplasia alter ribosome biogenesis and p53 levels. Life science alliance   3(8) Full text @ Life Sci Alliance
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Figure 3. <italic>exosc8</italic> and <italic>exosc9</italic> homozygous mutant zebrafish develop cerebellar atrophy.

(A) Immunofluorescence in 5-dpf wild-type, exosc8 homozygous mutant and exosc9 homozygous mutant zebrafish embryos with antibodies raised against HuC (green) and Pvalb7 (red) Pvalb7 is a marker for Purkinje cells in the cerebellum, HuC is an early neuronal marker. Scale bar: 500 μm. (B, C) Quantification of (B) HuC-positive area and (C) Pvalb7-positive area in 5-dpf wild-type, exosc8 homozygous mutant and exosc9 homozygous mutant zebrafish embryos. Three control, six exosc8 (c.26_27del), and six exosc9 (c.198_208del) homozygous larvae were measured for the HuC quantification. 6 control, 7 exosc8 (c.26_27del), and 11 exosc9 (c.198_208del) homozygous larvae were measured for the Pvalb7 quantification. Error bars represent the standard error (±SEM) and statistical analysis was performed using unpaired t tests (exosc8 versus wt and exosc9 versus wt, respectively). Labelling: C, cerebellum; F, forebrain; H, hindbrain and spinal cord; M, midbrain.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
exosc8cu201/cu201 standard conditions Day 5 cerebellum decreased size, abnormal
Day 5 midbrain decreased size, abnormal
exosc9cu202/cu202 standard conditions Day 5 cerebellum decreased size, abnormal
Day 5 midbrain decreased size, abnormal
Acknowledgments:
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