FIGURE

Figure S5.

ID

ZDB-FIG-200622-26

Publication

Müller et al., 2020 - RNA exosome mutations in pontocerebellar hypoplasia alter ribosome biogenesis and p53 levels

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Figure S5.

Example of conversion of fibroblasts to induced neuronal progenitor cells (iNPCs) and qPCR.

(A, B, C, D, E, F) Cell morphology at different stages of the direct conversion procedure. (A) Day 0 fibroblasts before infection with Sendai virus. (B) Day 2 after Sendai virus infection and switch to neural induction medium. (C) Day 9. (D) Day 16. (E) Day 21. (F) Day 29. All scale bars: 50 μm. (G) qPCR to characterise expression of fibroblast, stem cell, and neuronal markers in iNPC colonies; the cells shown in this example are the EXOSC3 iNPCs. Expression levels were normalised to GAPDH and the respective fibroblast line (here EXOSC3). The neural stem cell markers were Sox2, Sox1, nestin, and Pax6; the fibroblast markers were Col1a1 and Col3a1. (H) Protein levels of control and patient fibroblasts and iNPCs were also analysed by immunoblotting using antibodies specific for EXOSC9, p53, and karyopherin or GAPDH as loading controls.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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