ZFIN ID: ZDB-FIG-200622-24
Müller et al., 2020 - RNA exosome mutations in pontocerebellar hypoplasia alter ribosome biogenesis and p53 levels. Life science alliance   3(8) Full text @ Life Sci Alliance
Observed In:
Stage: Long-pec

Figure 4. <italic>exosc8</italic> and <italic>exosc9</italic> homozygous mutant zebrafish have increased apoptosis.

(A) Acridine orange staining performed on 48-hpf wild-type, exosc8 homozygous mutant and exosc9 homozygous mutant zebrafish embryos. Representative images of each category are shown here. Four different clutches of offspring with 24 embryos each have been analysed; the acridine orange staining has been performed and evaluated first (without knowledge of the genotype), followed by genotyping of all embryos that were stained. Scale bar: 500 μm. (B) Quantification of acridine orange positive spots. Spots were counted in 36 images of wild-type or heterozygous embryos and 18 images of homozygous mutant embryos. Bright spots in the yolk sac were not counted. Error bars represent the standard error (±SEM) and statistical analysis was performed using the unpaired t test. ****P < 0.0001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
exosc8cu201/cu201 standard conditions Long-pec head apoptotic process increased occurrence, abnormal
exosc9cu202/cu202 standard conditions Long-pec head apoptotic process increased occurrence, abnormal
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Life Sci Alliance