ZFIN ID: ZDB-FIG-200622-24
Müller et al., 2020 - RNA exosome mutations in pontocerebellar hypoplasia alter ribosome biogenesis and p53 levels. Life science alliance   3(8) Full text @ Life Sci Alliance
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Figure 4. <italic>exosc8</italic> and <italic>exosc9</italic> homozygous mutant zebrafish have increased apoptosis.

(A) Acridine orange staining performed on 48-hpf wild-type, exosc8 homozygous mutant and exosc9 homozygous mutant zebrafish embryos. Representative images of each category are shown here. Four different clutches of offspring with 24 embryos each have been analysed; the acridine orange staining has been performed and evaluated first (without knowledge of the genotype), followed by genotyping of all embryos that were stained. Scale bar: 500 μm. (B) Quantification of acridine orange positive spots. Spots were counted in 36 images of wild-type or heterozygous embryos and 18 images of homozygous mutant embryos. Bright spots in the yolk sac were not counted. Error bars represent the standard error (±SEM) and statistical analysis was performed using the unpaired t test. ****P < 0.0001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
exosc8cu201/cu201 standard conditions Long-pec head apoptotic process increased occurrence, abnormal
exosc9cu202/cu202 standard conditions Long-pec head apoptotic process increased occurrence, abnormal
Acknowledgments:
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