ZFIN ID: ZDB-FIG-200622-23
Müller et al., 2020 - RNA exosome mutations in pontocerebellar hypoplasia alter ribosome biogenesis and p53 levels. Life science alliance   3(8) Full text @ Life Sci Alliance
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Figure S4. <italic>exosc8</italic> and <italic>exosc9</italic> homozygous mutant zebrafish develop muscular atrophy.

Immunofluorescence in 5-dpf wild-type, exosc8 homozygous mutant, and exosc9 homozygous mutant zebrafish larvae with phalloidin conjugated to Alexa Fluor 594. Phalloidin staining at 5 dpf showed that the exosc8 (c.26_27del) and exosc9 (c.198_208del) homozygous larvae appeared to have thinner tails than WT and heterozygous clutchmates (representative images shown here). Muscle fibres appeared disorganized and not straight with gaps between fibres in the exosc8 (c.26_27del) and exosc9 (c.198_208del) homozygous larvae compared with WT and heterozygous clutchmates. Scale bar: 50 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
exosc8cu201/cu201 standard conditions Day 5 caudal fin decreased thickness, abnormal
Day 5 skeletal muscle cell disorganized, abnormal
exosc9cu202/cu202 standard conditions Day 5 caudal fin decreased thickness, abnormal
Day 5 skeletal muscle cell disorganized, abnormal
Acknowledgments:
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