ZFIN Figure: Müller et al., 2020, Figure S4.

ZFIN ID: ZDB-FIG-200622-23

ZFIN ID: ZDB-FIG-200622-23

Müller et al., 2020 - RNA exosome mutations in pontocerebellar hypoplasia alter ribosome biogenesis and p53 levels. Life science alliance 3(8) Full text @ Life Sci Alliance

ADDITIONAL FIGURES

PHENOTYPE:

Fish:	exosc8^cu201/cu201 exosc9^cu202/cu202
Observed In:	caudal fin skeletal muscle cell
Stage:	Day 5

Figure S4. <italic>exosc8</italic> and <italic>exosc9</italic> homozygous mutant zebrafish develop muscular atrophy.

Immunofluorescence in 5-dpf wild-type, exosc8 homozygous mutant, and exosc9 homozygous mutant zebrafish larvae with phalloidin conjugated to Alexa Fluor 594. Phalloidin staining at 5 dpf showed that the exosc8 (c.26_27del) and exosc9 (c.198_208del) homozygous larvae appeared to have thinner tails than WT and heterozygous clutchmates (representative images shown here). Muscle fibres appeared disorganized and not straight with gaps between fibres in the exosc8 (c.26_27del) and exosc9 (c.198_208del) homozygous larvae compared with WT and heterozygous clutchmates. Scale bar: 50 μm.

Gene Expression Details No data available

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
exosc8^cu201/cu201	standard conditions	Day 5	caudal fin decreased thickness, abnormal
		Day 5	skeletal muscle cell disorganized, abnormal
exosc9^cu202/cu202	standard conditions	Day 5	caudal fin decreased thickness, abnormal
		Day 5	skeletal muscle cell disorganized, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Life Sci Alliance