Figure 8—figure supplement 1.

Photocurrent amplitude and kinetics as a function of irradiance.

(A) Example GtACR1 photocurrents obtained by providing a 1 s light periods at different holding potentials (Vhold) using intracellular solutions approximating either embryonic or larval ECl. Orange traces denote holding potentials closest to ECl. (B) GtACR1 photocurrent I-V curves (mean ± SD). Photocurrents reverse with a positive 5–10 mV shift relative to ECl (dotted lines) in both solutions. (C) Example photocurrents from an eNpHR3.0-expressing cell at different irradiance levels (3–20 mW/mm2). (D,E) Photocurrent peak (D) and steady-state (E) amplitude vs. irradiance (mean ± SEM, across cells). Asterisks indicate a significant non-zero slope. F–H Photocurrent activation (F), inactivation (G) and deactivation (H) time constants vs. irradiance (mean ± SEM). eNpHR3.0 photocurrents did not inactivate hence no inactivation time constant was computed.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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