Figure 5—figure supplement 1.

Optogenetically-evoked voltage responses as a function of irradiance and pulse frequency.

(A) Fraction of cells that generated spikes in response to single light pulses (0.1–5 ms). (B) Peak depolarisation across irradiance levels (1–30 mW/mm2; mean ± SEM, across cells). Orange line indicates threshold for spike detection (–30 mV). (C) Time to peak depolarisation (mean ± SEM). (D) Number of evoked spikes vs. irradiance (1–5 ms pulse duration). In CoChR-expressing cells, 2–5 ms light pulses induced spike bursts (mean ± SEM). (E) Spike latency vs. pulse number (mean ± SEM). With increasing pulse frequency, CoChR-expressing cells showed progressively longer spike latency throughout the pulse train.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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