ZFIN ID: ZDB-FIG-200129-28
Yang et al., 2019 - Omics-based Investigation of Diet-induced Obesity Synergized with HBx, Src, and p53 Mutation Accelerating Hepatocarcinogenesis in Zebrafish Model. Cancers   11(12) Full text @ Cancers
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Figure 2

Expression of selected markers in various genetic background zebrafish fed with different diets. Expression of lipogenic enzymes (agpat, fasn, and pap), lipogenic factors (pparg, srebf1, and chrebp), and cell cycle/proliferation-related genes (ccne1, cdk1, and cdk2) in (AC) WT, (DF) HBx(p53-), (GI) Src(p53-), and (JL) HBx,Src(p53-) fish after eight weeks of normal diet (NOR), overfeeding (diet-induced obesity, DIO), or high-fat diet (FAT). The number of fish is 20 for each group, and the number of experimental replicates for qPCR analysis is three. Expression fold change compared to WT_NOR control. Statistical analysis of results was performed using a two-tailed Student’s t-test. Asterisks (*) represent level of significance: * p-value ≤ 0.05; ** p-value ≤ 0.01; *** p-value ≤ 0.001; **** p-value ≤ 0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
agpat4 AB high fat Adult liver RTPCR
ccne1 AB high fat Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg high fat Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1003Tg increased food availability Adult liver RTPCR
cdk1 AB increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg high fat Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg increased food availability Adult liver RTPCR
cdk2 AB increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg high fat Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1003Tg high fat Adult liver RTPCR
fasn AB increased food availability Adult liver RTPCR
AB high fat Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg high fat Adult liver RTPCR
tp53zdf1/zdf1; nn1003Tg increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1003Tg high fat Adult liver RTPCR
mlxip AB increased food availability Adult liver RTPCR
AB high fat Adult liver RTPCR
tp53zdf1/zdf1; nn1003Tg high fat Adult liver RTPCR
pparg AB increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1003Tg increased food availability Adult liver RTPCR
srebf1 AB increased food availability Adult liver RTPCR
AB high fat Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg high fat Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1003Tg increased food availability Adult liver RTPCR
tp53zdf1/zdf1; nn1003Tg high fat Adult liver RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB increased food availability Adult liver cdk1 expression increased amount, abnormal
Adult liver cdk2 expression decreased amount, abnormal
Adult liver fasn expression decreased amount, abnormal
Adult liver mlxip expression decreased amount, abnormal
Adult liver pparg expression increased amount, abnormal
Adult liver srebf1 expression increased amount, abnormal
AB high fat Adult liver agpat4 expression increased amount, abnormal
Adult liver ccne1 expression decreased amount, abnormal
Adult liver fasn expression increased amount, abnormal
Adult liver mlxip expression decreased amount, abnormal
Adult liver srebf1 expression increased amount, abnormal
tp53zdf1/zdf1; nn1002Tg increased food availability Adult liver pparg expression increased amount, abnormal
Adult liver srebf1 expression increased amount, abnormal
tp53zdf1/zdf1; nn1002Tg high fat Adult liver ccne1 expression decreased amount, abnormal
Adult liver cdk1 expression decreased amount, abnormal
Adult liver cdk2 expression increased amount, abnormal
Adult liver fasn expression increased amount, abnormal
Adult liver srebf1 expression increased amount, abnormal
tp53zdf1/zdf1; nn1002Tg; nn1003Tg increased food availability Adult liver ccne1 expression increased amount, abnormal
Adult liver cdk1 expression increased amount, abnormal
Adult liver cdk2 expression increased amount, abnormal
Adult liver srebf1 expression increased amount, abnormal
tp53zdf1/zdf1; nn1003Tg increased food availability Adult liver ccne1 expression decreased amount, abnormal
Adult liver fasn expression decreased amount, abnormal
Adult liver pparg expression decreased amount, abnormal
Adult liver srebf1 expression decreased amount, abnormal
tp53zdf1/zdf1; nn1003Tg high fat Adult liver cdk2 expression decreased amount, abnormal
Adult liver fasn expression decreased amount, abnormal
Adult liver mlxip expression increased amount, abnormal
Adult liver srebf1 expression increased amount, abnormal
Acknowledgments:
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