ZFIN ID: ZDB-FIG-191230-1552
Sucharov et al., 2019 - Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway. PLoS Genetics   15:e1008507 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig 10 <italic>jag1b</italic> is similarly regulated between low- and high-penetrance strains, but the <italic>jag1b</italic> transcriptional target <italic>her6</italic> exhibits strain-specific regulation.

(A) Head expression of jag1b was quantified by RT-qPCR in full-sibling mef2ca mutant and wild-type embryos from low- and high-penetrance strains at 28 and 48 hpf. Expression of jag1b in mef2ca mutants was normalized to wild-type sibling expression levels (black line). Asterisks mark expression levels significantly different in mef2ca mutants compared with mef2ca wild types (T-test at alpha = 0.05), error bars are standard deviation. (B) Head expression of the Jag/N transcriptional targets hey1 and her6 was quantified by RT-qPCR in full-sibling jag1b mutant and wild-type embryos at 28 and 48 hpf. Expression of each target in jag1b mutants was normalized to wild-type sibling expression levels (black line). Asterisks mark genes with expression levels significantly different in jag1b mutants compared with jag1b wild types (T-test at alpha = 0.05). Error bars are standard deviation. (C) Head expression of the Jag/N transcriptional targets hey1 and her6 was quantified by RT-qPCR in full-sibling mef2ca mutant and wild-type embryos from low- and high-penetrance strains at 28 and 48 hpf. Expression of each gene in mef2ca mutants was normalized to wild-type sibling expression levels (black line). Asterisks mark genes with expression levels significantly different in mef2ca mutants compared with mef2ca wild types (T-test at alpha = 0.05). Error bars are standard deviation. (D) Spatial expression of her6 was determined by in situ hybridization. her6 is expressed in three discrete patches in pharyngeal arch 2 (arrows) in low-penetrance wild types, low-penetrance mutants and high-penetrance wild types. In only high-penetrance mef2ca mutants her6 expands ventrally (arrowhead). dlx2a expression was used to delineate pharyngeal arches one (a1) and two (a2) (dashed outlines). In the high penetrance strain, her6 was expanded in 0/7 homozygous wild types and 7/8 homozygous mutants. In the low-penetrance strain, her6 was expanded in 0/3 homozygous wild types and 0/5 homozygous mutants. Scale bar: 30 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT standard conditions Long-pec pharyngeal arch 1 ISH
Long-pec pharyngeal arch 2 ISH
mef2cab1086/b1086 standard conditions Long-pec pharyngeal arch 1 ISH
Long-pec pharyngeal arch 2 ISH
her6 WT standard conditions Long-pec pharyngeal arch 2 ISH
jag1bb1105/b1105 standard conditions Prim-5 head RTPCR
Long-pec head RTPCR
mef2cab1086/b1086 standard conditions Prim-5 head RTPCR
Long-pec head RTPCR
Long-pec pharyngeal arch 2 ISH
hey1 jag1bb1105/b1105 standard conditions Prim-5 head RTPCR
Long-pec head RTPCR
mef2cab1086/b1086 standard conditions Prim-5 head RTPCR
Long-pec head RTPCR
jag1b mef2cab1086/b1086 standard conditions Prim-5 head RTPCR
Long-pec head RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jag1bb1105/b1105 standard conditions Prim-5 head her6 expression decreased amount, abnormal
Prim-5 head hey1 expression decreased amount, abnormal
Long-pec head her6 expression decreased amount, abnormal
Long-pec head hey1 expression decreased amount, abnormal
mef2cab1086/b1086 standard conditions Long-pec head her6 expression increased amount, abnormal
Long-pec head hey1 expression increased amount, abnormal
Long-pec head jag1b expression increased amount, abnormal
Long-pec pharyngeal arch 2 her6 expression increased distribution, abnormal
Acknowledgments:
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