ZFIN ID: ZDB-FIG-191230-1543
Sucharov et al., 2019 - Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway. PLoS Genetics   15:e1008507 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 6

Fig 2 Selective breeding just for ectopic bone penetrance modifies penetrance of other <italic>mef2ca</italic>-associated craniofacial phenotypes to different extents.

(A) Wild-type 6 days post fertilization (dpf) zebrafish larvae were stained with Alcian Blue and Alizarin Red to label cartilage and bone then flat mounted and imaged. The following craniofacial skeletal elements are indicated in the micrograph and graphic: opercle bone (op), branchiostegal ray (br), Meckel’s (mc), ceratohyal (ch), symplectic (sy) and hyomandibular (hm) cartilages, interhyal (ih) and jaw (jj) joints. Scale bar: 50 μm. (B, C) Penetrance of most phenotypes associated with mef2ca are reduced in homozygous mutants from the low-penetrance strain compared to homozygous mutants from the high-penetrance strain including: ectopic bone (arrowheads), interhyal- and jaw-joint fusions (^), dysmorphic ch (arrows), reduced mc (double arrowhead). A shortened sy cartilage (red arrows) remains fully penetrant in both strains. (D) Penetrance of mef2ca-associated phenotypes from full-sibling low- and high-penetrance families. Asterisks indicate statistically significant penetrance differences between homozygous mutants in low-penetrance compared with high-penetrance strains (Fisher’s exact test at alpha = 0.05).

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mef2cab1086/b1086 standard conditions Day 6 ceratohyal cartilage morphology, abnormal
Day 6 ceratohyal-interhyal joint fused with ceratohyal-interhyal joint, abnormal
Day 6 Meckel's cartilage decreased size, abnormal
Day 6 opercle mislocalised, abnormal
Day 6 quadrate-anguloarticular joint fused with quadrate-anguloarticular joint, abnormal
Day 6 symplectic decreased size, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.