ZFIN ID: ZDB-FIG-191230-1551
Sucharov et al., 2019 - Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway. PLoS Genetics   15:e1008507 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 6

Fig 9 Loss of <italic>jag1b</italic> function decreases the penetrance of <italic>mef2ca</italic>-associated phenotypes.

(A) Animals double heterozygous for mef2ca and jag1b were intercrossed, raised to 6 dpf and fixed and stained with Alcian Blue (cartilage) and Alizarin Red (bone). Genotyped animals were flat mounted and imaged. Removing functional copies of jag1b decreased mef2ca mutant penetrance. Scale bar: 50 μm. (B) Genotyped mef2ca;jag1b skeletal preparations were scored for penetrance of mef2ca-associated phenotypes. Asterisks indicate significance at alpha = 0.05 Fisher’s exact test compared with jag1b+/+. (C) Genotyped mef2ca;jag1b skeletal preparations were scored for penetrance of jag1b-associated phenotypes. Asterisks indicate significance at alpha = 0.05 Fisher’s exact test compared with mef2ca+/+.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jag1bb1105/+; mef2cab1086/b1086 standard conditions Day 6 ceratohyal cartilage morphology, ameliorated
Day 6 ceratohyal-interhyal joint fused with ceratohyal-interhyal joint, ameliorated
Day 6 Meckel's cartilage decreased size, ameliorated
Day 6 opercle mislocalised, ameliorated
Day 6 quadrate-anguloarticular joint fused with quadrate-anguloarticular joint, abnormal
Day 6 symplectic decreased size, abnormal
jag1bb1105/b1105 standard conditions Day 6 foramen increased size, abnormal
Day 6 opercle decreased size, abnormal
jag1bb1105/b1105; mef2cab1086/b1086 standard conditions Day 6 ceratohyal cartilage morphology, ameliorated
Day 6 ceratohyal-interhyal joint fused with ceratohyal-interhyal joint, ameliorated
Day 6 foramen increased size, ameliorated
Day 6 Meckel's cartilage decreased size, abnormal
Day 6 opercle decreased size, ameliorated
Day 6 opercle mislocalised, ameliorated
Day 6 quadrate-anguloarticular joint fused with quadrate-anguloarticular joint, abnormal
Day 6 symplectic decreased size, abnormal
mef2cab1086/b1086 standard conditions Day 6 ceratohyal cartilage morphology, abnormal
Day 6 ceratohyal-interhyal joint fused with ceratohyal-interhyal joint, abnormal
Day 6 Meckel's cartilage decreased size, abnormal
Day 6 opercle mislocalised, abnormal
Day 6 quadrate-anguloarticular joint fused with quadrate-anguloarticular joint, abnormal
Day 6 symplectic decreased size, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.