ZFIN ID: ZDB-FIG-191230-1546
Sucharov et al., 2019 - Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway. PLoS Genetics   15:e1008507 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Day 6

Fig 4 Selective breeding just for ectopic bone penetrance modifies the behavior of the <italic>mef2ca</italic><sup><italic>b1086</italic></sup> mutant allele.

(A) Low-penetrance mef2ca heterozygous adults were intercrossed and offspring were grown to adulthood. Fin amputation and genotyping identified mef2ca homozygous adults, which were imaged alongside wild-type siblings. Scale bar: 1mm. (B) 6 dpf full-sibling individuals from a single high-penetrance family were stained with Alizarin Red (bone) and Alcian Blue (cartilage), then genotyped and mef2ca heterozygotes and homozygous wild types were scored for presence of all mef2ca-associated phenotypes. (C) 6 dpf Alizarin Red and Alcian Blue stained skeletons were dissected and flat mounted, lateral view. In these individuals, a shortened symplectic (sy) cartilage phenotype is evident in mef2ca heterozygotes from the high-penetrance strain and mef2ca homozygous mutants from the low-penetrance strain. Scale bar: 50 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mef2cab1086/+ standard conditions Day 6 symplectic decreased size, abnormal
mef2cab1086/b1086 standard conditions Day 6 symplectic decreased size, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.