ZFIN ID: ZDB-FIG-191230-1546
Sucharov et al., 2019 - Selective breeding modifies mef2ca mutant incomplete penetrance by tuning the opposing Notch pathway. PLoS Genetics   15:e1008507 Full text @ PLoS Genet.
Observed In:
Stage: Day 6

Fig 4 Selective breeding just for ectopic bone penetrance modifies the behavior of the <italic>mef2ca</italic><sup><italic>b1086</italic></sup> mutant allele.

(A) Low-penetrance mef2ca heterozygous adults were intercrossed and offspring were grown to adulthood. Fin amputation and genotyping identified mef2ca homozygous adults, which were imaged alongside wild-type siblings. Scale bar: 1mm. (B) 6 dpf full-sibling individuals from a single high-penetrance family were stained with Alizarin Red (bone) and Alcian Blue (cartilage), then genotyped and mef2ca heterozygotes and homozygous wild types were scored for presence of all mef2ca-associated phenotypes. (C) 6 dpf Alizarin Red and Alcian Blue stained skeletons were dissected and flat mounted, lateral view. In these individuals, a shortened symplectic (sy) cartilage phenotype is evident in mef2ca heterozygotes from the high-penetrance strain and mef2ca homozygous mutants from the low-penetrance strain. Scale bar: 50 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mef2cab1086/+ standard conditions Day 6 symplectic decreased size, abnormal
mef2cab1086/b1086 standard conditions Day 6 symplectic decreased size, abnormal
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