ZFIN ID: ZDB-FIG-190627-36
Yildiz et al., 2019 - Zebrafish prdm12b acts independently of nkx6.1 repression to promote eng1b expression in the neural tube p1 domain. Neural Development   14:5 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Days 45-89

Fig. 7

prdm12b does not maintain the p1 domain by repressing nkx6.1. a. Anti-Nkx6.1 immunostaining of nkx6.1um321/um321 mutant (left) and wildtype (right) embryos at 30hpf. b. Chart indicating the frequency of each genotype at various time points in broods from crosses of nkx6.1+/um321 heterozygous mutants. c. Hb9 immunostaining in wildtype (left) versus a cross of nkx6.1+/um321 heterozygous embryos (right) at 33hpf. d. Islet-1/2 immunostaining of 50hpf embryos from a cross of nkx6.1+/um321 heterozygotes. e. Expression of eng1b in 24hpf embryos from a cross of nkx6.1+/um321 heterozygotes. f. Expression of eng1b in 24hpf uninjected wildtype embryos (left panels), 24hpf prdm12b MO-injected wildtype embryos (middle panels) and 24hpf prdm12b MO-injected embryos from a cross of nkx6.1+/um321heterozygotes (right panels). g. Expression of prdm12b in a representative wildtype embryo (left panel) and a representative embryo from a cross of nkx6.1+/um321 heterozygotes (middle panel) at 24hpf. Right panel shows quantification of the size of the prdm12b expression domain in 11 wildtype embryos and 20 embryos from a cross of nkx6.1+/um321 heterozygotes. Numbers in panels indicate the fraction of embryos displaying the phenotype shown

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
en1b WT standard conditions Prim-5 CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 MiV1 ISH
Prim-5 somite ISH
WT + MO1-prdm12b standard conditions Prim-5 Not Detected CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 Not Detected MiV1 ISH
Prim-5 somite ISH
nkx6.1um321/um321 standard conditions Prim-5 CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 MiV1 ISH
Prim-5 somite ISH
nkx6.1um321/um321 + MO1-prdm12b standard conditions Prim-5 Not Detected CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 Not Detected MiV1 ISH
Prim-5 somite ISH
prdm12b WT standard conditions Prim-5 spinal cord ventral region ISH
nkx6.1um321/um321 standard conditions Prim-5 spinal cord ventral region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mnx1 IHC WT standard conditions Prim-15 motor neuron
IHC nkx6.1um321/um321 standard conditions Prim-15 motor neuron
Ab2-isl IHC WT standard conditions Long-pec branchiomotor neuron
IHC nkx6.1um321/um321 standard conditions Long-pec branchiomotor neuron
Phenotype Details
Fish Conditions Stage Phenotype
nkx6.1um321/um321 standard conditions Long-pec branchiomotor neuron morphology, abnormal
Days 45-89 whole organism decreased life span, abnormal
Acknowledgments:
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