ZFIN ID: ZDB-FIG-190627-33
Yildiz et al., 2019 - Zebrafish prdm12b acts independently of nkx6.1 repression to promote eng1b expression in the neural tube p1 domain. Neural Development   14:5 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 256-cell to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 4 to Days 14-20

Fig. 2

prdm12b germ line mutants lack eng1b expression in the p1 domain. a, b. prdm12b is not maternally deposited. In situ hybridization detects prdm12b expression at 24hpf (b), but not at 2.5hpf (a), in wildtype embryos. c. Bar chart depicting the frequency of each genotype at various time points in broods from crosses of prdm12b heterozygous animals. Error bars indicate ±S. E. (n = 3). dpf = days post fertilization, mo = months. d, e. Morphology of 15dpf prdm12b+/+ (d) and prdm12bum319/um319 (e) fish. f-s. eng1b expression in 24hpf embryos from crosses of prdm12b+/um318 heterozygotes (f-k), or prdm12b+/um319 heterozygotes (l-s). Numbers in each panel indicate the fraction of animals with the specified phenotype. t, u. evx1expression in 24hpf embryos from a cross of prdm12b+/um319 heterozygotes. v, w. vsx2expression in 24hpf embryos from a cross of prdm12b+/um319 heterozygotes. Embryos are shown in dorsal (f-h, l-n, t-y) or lateral (i-k, o-q) view with anterior to the left, or in cross section (r, s) with dorsal to the top. Brackets indicate r4, arrows mark V1 interneurons and arrowheads mark somites. MHB = midbrain–hindbrain boundary, HB = hindbrain and SC = spinal cord

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
en1b WT standard conditions Prim-5 CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 MiV1 ISH
Prim-5 somite ISH
prdm12bum318/um318 standard conditions Prim-5 Not Detected CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 Not Detected MiV1 ISH
Prim-5 somite ISH
prdm12bum319/um319 standard conditions Prim-5 Not Detected CiA ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 Not Detected MiV1 ISH
Prim-5 somite ISH
evx1 WT standard conditions Prim-5 hindbrain ventral region ISH
prdm12bum319/um319 standard conditions Prim-5 hindbrain ventral region ISH
prdm12b WT standard conditions 256-cell Not Detected whole organism ISH
Prim-5 whole organism ISH
vsx2 WT standard conditions Prim-5 hindbrain ventral region ISH
prdm12bum319/um319 standard conditions Prim-5 hindbrain ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
prdm12bum318/um318 standard conditions Day 4 - Days 14-20 whole organism decreased life span, abnormal
prdm12bum319/um319 standard conditions Day 4 - Days 14-20 whole organism decreased life span, abnormal
Acknowledgments:
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