ZFIN Figure: Blanco-Sánchez et al., 2018, Fig. 5

ZFIN ID: ZDB-FIG-190107-3

ZFIN ID: ZDB-FIG-190107-3

Blanco-Sánchez et al., 2018 - Grxcr1 Promotes Hair Bundle Development by Destabilizing the Physical Interaction between Harmonin and Sans Usher Syndrome Proteins. Cell Reports 25:1281-1291.e4 Full text @ Cell Rep.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	grxcr1a
Antibodies:	Ab1-golga2 Ab1-grxcr1a Ab3-hspa5
Fish:	WT
Anatomical Terms:	hair cell anterior macula cell body hair cell anterior macula cis-Golgi network hair cell anterior macula endoplasmic reticulum hair cell anterior macula endoplasmic reticulum-Golgi intermediate compartment hair cell anterior macula kinocilium hair cell anterior macula stereocilium
Stage:	Day 5

Fig. 5

Grxcr1 Localizes in the Golgi Apparatus and ERGIC of the Hair Cells

(A–C) Confocal sections of the anterior macula. Shown are partial colocalization of Grxcr1 (red) and phalloidin (A), GM130 (B), or KDEL (green, C). Individual hair cells are outlined in white. Blue, red, and yellow arrows show Grxcr1 localization in the cell body, hair bundles, and kinocilia, respectively. The confocal sections were selected based on the localization of the organelles of interest (hair bundles, Golgi, and ER), hence the differences in Grxcr1 localization. Nuclei are outlined in blue; 5 dpf.

Gene Expression Details

Gene	Antibody	Fish	Conditions	Stage	Anatomy	Assay
grxcr1a	Ab1-grxcr1a	WT	standard conditions	Day 5	hair cell anterior macula cell body	IHC
	Ab1-grxcr1a			Day 5	hair cell anterior macula kinocilium	IHC
	Ab1-grxcr1a			Day 5	hair cell anterior macula stereocilium	IHC

Antibody Labeling Details

Antibody	Assay	Fish	Conditions	Stage	Anatomy
Ab1-golga2	IHC	WT	standard conditions	Day 5	hair cell anterior macula cis-Golgi network
	IHC			Day 5	hair cell anterior macula endoplasmic reticulum-Golgi intermediate compartment
Ab3-hspa5	IHC	WT	standard conditions	Day 5	hair cell anterior macula endoplasmic reticulum

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.