ZFIN ID: ZDB-FIG-190107-8
Blanco-Sánchez et al., 2018 - Grxcr1 Promotes Hair Bundle Development by Destabilizing the Physical Interaction between Harmonin and Sans Usher Syndrome Proteins. Cell Reports   25:1281-1291.e4 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. S6

Grxcr1 antibody is specific, and anti-Ush1ga and commercial anti-Ush1g antibodies recognize Ush1ga but not Ush1gb. Related to Figures 5 and 6. (A) Schematic of Grxcr1 protein showing the epitope (outlined in red) recognized by the anti-Grxcr1 antibody. (B) MDCK cells were transfected with grxcr1-HA and immuno-labelled using anti-Grxcr1 and anti-HA antibodies. Grxcr1 was detected within transfected cells as shown by the co-localization between Grxcr1 (green) and HA (red). Background at the cell membrane was also observed. (C) MDCK cells were transfected with pcDNA3.1 and immuno-labelled using anti-Grxcr1 and anti-HA antibodies. Only background at the cell membrane could be detected. Immuno-labelling using anti-Grxcr1 antibody in grxcr1+/+ (D) and grxcr1-/- mutant larvae (D’) at 5 dpf. (E) Immuno-labeling using KDEL antibody in Tg[myo6b:kdel-crimson]b1319 larvae at 5 dpf. (F) Schematic of Ush1ga protein showing the epitopes recognized by the anti-Ush1ga antibody (orange line), the mouse anti-Ush1g antibody (red line), the goat anti-Ush1g antibody (blue dotted line) and the rabbit anti-Ush1g antibody (purple line). ANK domains are indicated in green and the SAM domain is shown in pink. (G) MDCK cells were transfected with ush1ga-GFP. Ush1ga was detected within transfected cells as shown by the co-localization between Ush1ga (red) and GFP (green) signals. Background was also observed in some untransfected cells. (H) MDCK cells were transfected with ush1gb-HA. Ush1ga (red) could only be detected at background level in transfected cells (green). (I) MDCK cells were transfected with ush1ga-GFP and immuno-labelled with rabbit anti-Ush1g and anti-GFP antibodies. Ush1ga was detected in transfected cells as shown by the co-localization between Ush1g (red) and GFP (green) signals. (J) MDCK cells were transfected with ush1gb-HA and immuno-labelled with rabbit anti-Ush1g and anti-GFP antibodies. Ush1gb could not be detected in transfected cells. (K) MDCK cells were transfected with ush1ga-GFP and immuno-labelled with goat and mouse anti-Ush1g antibodies and anti-GFP antibody. Ush1ga was detected in transfected cells as shown by the co-localization between Ush1g (red-goat and blue-mouse) and GFP (green) signals. (L) MDCK cells were transfected with ush1gb-HA and immuno-labelled with goat and mouse anti-Ush1g antibodies and anti-GFP antibody. Ush1gb could not be detected in transfected cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
Crimson b1319Tg standard conditions Day 5 hair cell anterior macula IFL
grxcr1a Ab1-grxcr1a WT standard conditions Day 5 hair cell anterior macula IHC
Ab1-grxcr1a b1319Tg standard conditions Day 5 hair cell anterior macula IHC
Ab1-grxcr1a grxcr1ab1259/b1259 standard conditions Day 5 Not Detected hair cell anterior macula IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
grxcr1ab1259/b1259 standard conditions Day 5 hair cell anterior macula grxcr1a expression absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.