ZFIN ID: ZDB-FIG-190107-4
Blanco-Sánchez et al., 2018 - Grxcr1 Promotes Hair Bundle Development by Destabilizing the Physical Interaction between Harmonin and Sans Usher Syndrome Proteins. Cell Reports   25:1281-1291.e4 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 6

The Integrity of the cis-Golgi and Abundance of Ush1c and Ush1ga Are Affected in grxcr1−/− Mutants

(A and B) Immunolabeling of GM130 in the anterior maculae of grxcr1+/+ (A) and grxcr1−/− (B).

(C) Quantification of GM130 signal intensity in grxcr1+/+ (n = 29 larvae) and grxcr1−/− (n = 29 larvae) anterior maculae.

(D and E) Immunolabeling of Ush1c in the anterior maculae of grxcr1+/+ (D) and grxcr1−/− larvae (E).

(F) Quantification of Ush1c signal intensity in the anterior macula of grxcr1+/+ (n = 37 larvae) and grxcr1−/− larvae (n = 37 larvae).

(G and H) Immunolabeling of Ush1ga in the anterior maculae of grxcr1+/+ (G) and grxcr1−/− larvae (H).

(I) Quantification of Ush1ga signal intensity in grxcr1+/+ (n = 33 larvae) and grxcr1−/− larvae (n = 36 larvae).

(J and K) Immunolabeling of Ush1g in the anterior maculae of grxcr1+/+ (J) and grxcr1−/− larvae (K).

(L) Quantification of Ush1g signal intensity in grxcr1+/+ (n = 14 larvae) and grxcr1−/− larvae (n = 15 larvae).

Data were normalized to grxcr1+/+ and are represented as mean ± SEM. p < 0.05; 5 dpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ush1ga WT standard conditions Day 5 hair cell anterior macula IHC
Ab1-ush1ga Day 5 hair cell anterior macula IHC
Ab1-ush1ga grxcr1ab1259/b1259 standard conditions Day 5 hair cell anterior macula IHC
Day 5 hair cell anterior macula IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-golga2 IHC WT standard conditions Day 5 hair cell anterior macula
IHC grxcr1ab1259/b1259 standard conditions Day 5 hair cell anterior macula
Ab1-ush1ga IHC WT standard conditions Day 5 hair cell anterior macula
IHC grxcr1ab1259/b1259 standard conditions Day 5 hair cell anterior macula
Ab5-ush1c IHC WT standard conditions Day 5 hair cell anterior macula
IHC grxcr1ab1259/b1259 standard conditions Day 5 hair cell anterior macula
Phenotype Details
Fish Conditions Stage Phenotype
grxcr1ab1259/b1259 standard conditions Day 5 hair cell anterior macula ush1ga expression decreased amount, abnormal
Day 5 hair cell anterior macula ab1-golga2 labeling increased amount, abnormal
Day 5 hair cell anterior macula Ab1-ush1ga labeling decreased amount, abnormal
Day 5 hair cell anterior macula Ab5-ush1c labeling increased amount, abnormal
Day 5 hair cell anterior macula Golgi apparatus functionality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.