FIGURE

Fig. 6

ID
ZDB-FIG-180608-87
Publication
Dasgupta et al., 2018 - Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer's vesicle
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

Asymmetric cell shape changes in KV are separable from lumen expansion.

(A) Mosaic labeled KV cells in control MO injected embryos showed asymmetric changes in cell volumes and cell shapes between at 2 ss and 8 ss. (B–C) Perturbing cell-cell junction integrity in KV by interfering with jupa (B) or lgl2 (C) expression inhibited KV lumen expansion, but asymmetric cell volume changes occurred that were similar to controls. In addition, asymmetric KV cell shape changes occurred normally in jupa and lgl2 MO embryos. (D) Quantification of 3D reconstructed KV lumen volumes (insets depict KV lumen in YZ axis) in control and treated live embryos at 8 ss. For quantitative analyses, the mean + SD is shown. The number of KV-ant and KV-post cells analyzed is indicated in the graphs in A-C. N = number of embryos analyzed. Data for control MO and jupa MO experiments are pooled from three independent experiments and lgl2 MO data are pooled from two experiments. Scale = 20 μm. *p<0.01 and ns = not significant (p>5% with Welch’s T-Test).

Expression Data
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 1-4 somites to 5-9 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: 5-9 somites

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife