FIGURE

Fig. S7

ID

ZDB-FIG-170927-11

Publication

Feng et al., 2017 - Optical Control of Tumor Induction in the Zebrafish

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Fig. S7

Constitutive activation of kRASG12V in transgenic fish lines. (A) Cyclofen was not able to induce mTFP expression in the heterozygous Tg(ubi:loxP-Eos-stop-kRASG12V-T2AmTFP; ubi:Cre-ERT2) embryo. (B) Oncogene activation could be observed in a Tg(ubi:loxPEos- stop-kRASG12V-T2A-mTFP) injected with Cre-ERT2 mRNA: distinct mTFP expression patterns in non-activated, leaky (see arrows) and photo-activated embryos are shown. (C) Success rate of two-photon activation of the oncogene (via the fluorescence of the reporter protein, mTFP) in 19 embryos. (D) Quantification of two-photon activated kRASG12V signal compared to non-activated and cyclofen-activated mTFP/EosFP fluorescent ratios. Data are presented as mean ± SEM. **p < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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