IMAGE

Fig. S7

ID
ZDB-IMAGE-170927-11
Source
Figures for Feng et al., 2017
Image
Figure Caption

Fig. S7

Constitutive activation of kRASG12V in transgenic fish lines. (A) Cyclofen was not able to induce mTFP expression in the heterozygous Tg(ubi:loxP-Eos-stop-kRASG12V-T2AmTFP; ubi:Cre-ERT2) embryo. (B) Oncogene activation could be observed in a Tg(ubi:loxPEos- stop-kRASG12V-T2A-mTFP) injected with Cre-ERT2 mRNA: distinct mTFP expression patterns in non-activated, leaky (see arrows) and photo-activated embryos are shown. (C) Success rate of two-photon activation of the oncogene (via the fluorescence of the reporter protein, mTFP) in 19 embryos. (D) Quantification of two-photon activated kRASG12V signal compared to non-activated and cyclofen-activated mTFP/EosFP fluorescent ratios. Data are presented as mean ± SEM. **p < 0.01.

Acknowledgments
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