ZFIN ID: ZDB-FIG-170914-54
Askary et al., 2017 - Genome-wide analysis of facial skeletal regionalization in zebrafish. Development (Cambridge, England)   144(16):2994-3005 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-25

Fig. 4

Changes in domain-specific gene expression in edn1 and jag1b mutants. Two-color fluorescent in situ hybridizations were performed for genes of interest (red) and dlx2a (green) to label arch CNCCs at 36 hpf. Numbers indicate the gene expression fold-change in mutant versus wild-type fli1a:GFP+; sox10:dsRed+ CNCCs as determined by RNAseq. Maximum intensity projections show the mandibular (left) and hyoid (right) arches. (A) The ventral-intermediate expression of fgfbp2a, shox and stmn1a is lost in edn1 mutants, yet pouch expression of stmn1a is unaffected. (B) In edn1 mutants, intermediate expression of fsta and ctgfb is lost, emx2 is variably upregulated or downregulated, and CNCC and mesoderm expression of igfbp5b is unaltered. We also note some ectopic ventral hyoid fsta expression in edn1 mutants. (C) Ventral expression of smad6a, skp2 and fzd9b is normal in edn1 mutants, yet ventral expression of pitx1 and sema3bl is lost. Note that dorsal expression of sema3bl is unaffected. (D) In jag1b mutants, dorsal expression of cd248a is lost, pou3f3a and pou3f3b are reduced, and cdh11 and kctd15a are unaffected. In edn1 mutants, cdh11 expression shifts to ventral CNCCs, pou3f3a and pou3f3b are ectopically expressed in ventral CNCCs, and cd248a is largely unaffected. Arrows indicate expression in predicted arch domains, open arrowheads indicate additional expression domains, and double arrows show expansion into other CNCC domains in mutants. Unless stated otherwise, consistent expression patterns were seen in a minimum of three wild types and three mutants for each experiment. Scale bars: 20 μm. (E) Summary of verified gene expression changes in edn1 and jag1b mutants. Unaffected genes are not listed.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cd248a edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
jag1bb1105/b1105; el10Tg; y1Tg standard conditions Prim-25 Not Detected pharyngeal arch 1 dorsal region ISH
Prim-25 Not Detected pharyngeal arch 2 dorsal region ISH
cdh11 edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
Prim-25 pharyngeal arch 2 medial region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
jag1bb1105/b1105; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
ccn2b edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
dlx2a el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
emx2 el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
fgfbp2a edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 Not Detected pharyngeal arch 1 ventral region ISH
Prim-25 Not Detected pharyngeal arch 2 ventral region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
fsta edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
Prim-25 ventral hyoid arch ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
fzd9b edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
igfbp5b edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 2 medial region ISH
kctd15a el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
jag1bb1105/b1105; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
pitx1 edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 Not Detected pharyngeal arch 1 ventral-most region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
pou3f3a edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
Prim-25 pharyngeal arch 2 medial region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
jag1bb1105/b1105; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
pou3f3b edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 1 medial region ISH
Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
Prim-25 pharyngeal arch 2 medial region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
jag1bb1105/b1105; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 pharyngeal arch 2 dorsal region ISH
sema3bl edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region ISH
Prim-25 Not Detected pharyngeal arch 1 ventral region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
shox edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 Not Detected pharyngeal arch 1 ventral region ISH
Prim-25 Not Detected pharyngeal arch 2 ventral region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
skp2 edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
smad6a edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral-most region ISH
Prim-25 pharyngeal arch 2 ventral-most region ISH
stmn1a edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 Not Detected pharyngeal arch 1 ventral region ISH
Prim-25 Not Detected pharyngeal arch 2 ventral region ISH
Prim-25 pharyngeal pouch ISH
el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 ventral region ISH
Prim-25 pharyngeal arch 2 ventral region ISH
Prim-25 pharyngeal pouch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 medial region ccn2b expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 medial region cdh11 expression spatial pattern, abnormal
Prim-25 pharyngeal arch 1 medial region fsta expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 medial region pou3f3a expression increased distribution, abnormal
Prim-25 pharyngeal arch 1 medial region pou3f3b expression increased distribution, abnormal
Prim-25 pharyngeal arch 1 ventral region fgfbp2a expression absent, abnormal
Prim-25 pharyngeal arch 1 ventral region pou3f3a expression increased distribution, abnormal
Prim-25 pharyngeal arch 1 ventral region pou3f3b expression increased distribution, abnormal
Prim-25 pharyngeal arch 1 ventral region sema3bl expression absent, abnormal
Prim-25 pharyngeal arch 1 ventral region shox expression absent, abnormal
Prim-25 pharyngeal arch 1 ventral region stmn1a expression absent, abnormal
Prim-25 pharyngeal arch 1 ventral-most region pitx1 expression absent, abnormal
Prim-25 pharyngeal arch 2 medial region ccn2b expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 medial region cdh11 expression spatial pattern, abnormal
Prim-25 pharyngeal arch 2 medial region fsta expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 medial region pou3f3a expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 medial region pou3f3b expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 ventral region fgfbp2a expression absent, abnormal
Prim-25 pharyngeal arch 2 ventral region pou3f3a expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 ventral region pou3f3b expression increased distribution, abnormal
Prim-25 pharyngeal arch 2 ventral region shox expression absent, abnormal
Prim-25 pharyngeal arch 2 ventral region stmn1a expression absent, abnormal
Prim-25 ventral hyoid arch fsta expression mislocalised, abnormal
jag1bb1105/b1105; el10Tg; y1Tg standard conditions Prim-25 pharyngeal arch 1 dorsal region cd248a expression absent, abnormal
Prim-25 pharyngeal arch 1 dorsal region pou3f3a expression decreased amount, abnormal
Prim-25 pharyngeal arch 1 dorsal region pou3f3b expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 dorsal region cd248a expression absent, abnormal
Prim-25 pharyngeal arch 2 dorsal region pou3f3a expression decreased amount, abnormal
Prim-25 pharyngeal arch 2 dorsal region pou3f3b expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development