ZFIN ID: ZDB-FIG-170914-52
Askary et al., 2017 - Genome-wide analysis of facial skeletal regionalization in zebrafish. Development (Cambridge, England)   144(16):2994-3005 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Day 5

Fig. 1

Isolation of arch CNCCs for RNAseq. (A) Cells were sorted using transgenic lines that label different populations of arch CNCCs. fli1a:GFP and sox10:DsRed overlap throughout arch CNCCs (yellow); dlx5a:GFP and sox10:DsRed in CNCCs of ventral (V) to intermediate (I) domains, as well as some dorsal (D) domain CNCCs and the otic vesicle; and hand2:GFP and sox10:DsRed in ventral and more weakly in ventral-intermediate (VI) CNCCs [modified from Barske et al. (2016) and Medeiros and Crump (2012)]. (B) Breakdown of alignment results for each RNAseq experiment, showing the percentage of reads aligned to a unique site in the genome and whether both paired-end reads were aligned. The line graph shows the total number of reads acquired for each sample. (C) Dorsal, intermediate, ventral-intermediate, and ventral domains were defined by their relative enrichment levels (i.e. fold-change of TPM values) among hand2:GFP; sox10:DsRed, dlx5a:GFP; sox10:DsRed, and fli1a:GFP; sox10:DsRed cells. These domains correspond to specific parts of the arches at 36 hpf that give rise to the larval craniofacial skeleton at 5 dpf (shown for context in a fli1a:GFP; sox10:DsRed fish).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed dlx5aj1073aGt; el10Tg standard conditions Prim-25 inner ear IFL
Prim-25 pharyngeal arch 1 cranial neural crest IFL
Prim-25 pharyngeal arch 2 cranial neural crest IFL
el10Tg; pd24Tg standard conditions Prim-25 inner ear IFL
Prim-25 pharyngeal arch 1 cranial neural crest IFL
Prim-25 pharyngeal arch 2 cranial neural crest IFL
el10Tg; y1Tg standard conditions Prim-25 inner ear IFL
Prim-25 pharyngeal arch 1 cranial neural crest IFL
Prim-25 pharyngeal arch 2 cranial neural crest IFL
Day 5 inner ear IFL
Day 5 pharyngeal arch 1 cranial neural crest IFL
Day 5 pharyngeal arch 2 cranial neural crest IFL
EGFP dlx5aj1073aGt; el10Tg standard conditions Prim-25 pharyngeal arch 1 cranial neural crest IFL
Prim-25 pharyngeal arch 2 cranial neural crest IFL
el10Tg; pd24Tg standard conditions Prim-25 pharyngeal arch ectoderm IFL
Prim-25 pharyngeal arch 1 cranial neural crest IFL
Prim-25 pharyngeal arch 2 cranial neural crest IFL
el10Tg; y1Tg standard conditions Prim-25 pharyngeal vasculature IFL
Day 5 ocular blood vessel IFL
Day 5 pharyngeal vasculature IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development