FIGURE

Fig. 10

ID

ZDB-FIG-170621-18

Publication

Solek et al., 2017 - Lineage tracing of dlx1a/2a and dlx5a/6a expressing cells in the developing zebrafish brain

Other Figures

All Figure Page

Back to All Figure Page

Fig. 10

Most of the supernumerary mCherry expressing cells in juvenile and adult Tg(dlx5/6Cre;Switch)^5d brains do not express gad65/67a. (A-C) Double IHC of juvenile (45 dpf) brains for mCherry (red) and HuC/D (green). n=3 in two biological replicates. Examples of cells expressing mCherry and HuC/D in the telencephalic area, the retina and the PGZ are indicated by white arrows in A''-C'' and A'''-C'''. (D-F) ISH for gad65 and gad67a probes (green) coupled with immunohistochemistry against mCherry proteins (red) on cryosections of adult (>6 months post-fertilization) brains. Examples of cells expressing mCherry and gad65/67a in the PPa near the ventricular zone (E) and in the Hd (F) are indicated by white arrows in E'', E''', F'' and F'''. n=4 in two biological replicates. (G) ISH for gad65 and gad67a probes (green) coupled with immunohistochemistry against mCherry proteins (red) on cryosections of Tg(dlx1/2Cre;Switch)^24h juvenile brain showing modest expansion of mCherry cells in the PGZ. An example of a single cell that expresses both mCherry and gad65/67a is indicated by a white arrow in G', G'' and G'''. Scale bar: A-E: 50 µm; A'''-E''': 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 427(1), Solek, C.M., Feng, S., Perin, S., Weinschutz Mendes, H.C., Ekker, M., Lineage tracing of dlx1a/2a and dlx5a/6a expressing cells in the developing zebrafish brain, 131-147, Copyright (2017) with permission from Elsevier. Full text @ Dev. Biol.