Fig. S5

Tg(epi+β-globin:eGFP) and Tg(2PΔepi:eGFP) together recapitulate endogenous and1 expression zebrafish embryonic fin folds. (A-G) 2 d.p.f., (O-U) 7 d.p.f. Tg(epi+β-globin:eGFP) transgenic reporter line. (H-N) 2 d.p.f , (V-Zii) 7 d.p.f Tg(2PΔepi:eGFP) transgenic reporter line. Ectodermal (E-G) and mesenchymal (L-M) expression is present in the median fin fold at 2 d.p.f.. Only ectodermal expression is present in the pectoral fin fold at 2 d.p.f. (indicated by the white arrowhead) (C). Note again, the absence of ectodermal expression in the early pectoral fin fold (indicated by the yellow arrowhead) (B-D). Ectodermal (P-U) and mesenchymal (W-Zii) reporter expression is present in the pectoral and median fin fold at 7 d.p.f.. Together these two lines recapitulate and1 expression. Note the stronger mesenchymal expression in the posterior half of the pectoral fin fold (indicated by the red arrowhead) (X). Brightfield (B, E, I, L, P, S, W, Z), fluorescence (A, C, F, H, J, M, O, Q, T, V, X, Zi), and brightfield/fluorescence merged images (D, G, K, N, R, U, Y, Zii). FF, Fin Fold; ED, Endoskeletal Disc; A, Anterior; P, Posterior; D, Dorsal; V, Ventral. Scale bars: 200µm in A, H, O, V; 30µm in B-D, I-K; 50µm in P-R, W-Y, E-G, L-N, S-U, Z-Zii.