FIGURE

Fig. 6

ID

ZDB-FIG-160512-41

Publication

Montgomery et al., 2016 - Intraspinal serotonergic neurons consist of two, temporally distinct populations in developing zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Figure 6. Kolmer-Agduhr (KA) neurons are 5-HT immunoreactive at 48 and 72 hpf. The Et(-1.5hsp70l:Gal4-VP16)^s1003t and Tg(UAS-E1b:Kaede)^s1999t lines were crossed to express Kaede in KA neurons (green) of progeny, which were immunolabeled for 5-HT (red). The Et(-1.5hsp70l:Gal4-VP16)^s1003t line did not drive transgene expression in all KA neurons. A-C: Kaede expression (A), but not 5-HT antibody labeling (B, merge in C), was detected at 24 hpf (dashed lines represent the dorsal and ventral boundaries of the spinal cord, approximately the same location in all panels). D-F: A subset of ventral KA neurons (D) were labeled with 5-HT antibodies (E, merge in F; arrows indicate colocalization). G-I: 5-HT antibody labeling (H) of KA neurons (G) was faint at 72 hpf (arrows) and Kaede-negative cells were more strongly labeled with 5-HT antibodies (H, merge in I). J-L: Kaede expression (J) and 5-HT antibody labeling (K) did not overlap at 96 hpf (L). Scale bar = 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Neurobiol.