FIGURE

Fig. 5

ID

ZDB-FIG-160512-40

Publication

Montgomery et al., 2016 - Intraspinal serotonergic neurons consist of two, temporally distinct populations in developing zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

ISNs that express pet1:EGFP are morphologically distinct from those that do not express pet1:EGFP. A 64 hpf Tg(-3.2pet1:EGFP)^ne0214 larva was labeled with antibodies to 5-HT, mounted laterally, and a confocal Z-stack was collected through the mediolateral extent of the spinal cord. The morphological structure of ISN somas (red) was examined in a confocal projection restricted to the medial spinal cord (Ai-Ci, medial projection) and a projection through the entire width of the spinal cord (Aii-Cii; full projection). A: 5-HT was detected in cell bodies near the ventral boundary of the spinal cord (dashed line, same position in all panels) that possessed two distinct shapes; dorsoventrally elongated conical cell bodies with apical terminals (Ai, arrows) and round or ovoid cell bodies (Aii, white arrowheads) that each possessed a single, prominent projection (Aii, black arrowheads). Apical terminals of the conically-shaped cells were positioned near the central canal (represented by a dotted line, same position in all panels). B and C: Pet1:EGFP (B, green) was expressed in ISNs with ovoid (white arrowheads), but not conical somas (arrows; merge in C). Scale bar = 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Neurobiol.