FIGURE

Fig. 2

ID

ZDB-FIG-151102-11

Publication

Yu et al., 2015 - Single cell analysis of endothelial morphogenesis in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Design and validation of an expression cassette for analyzing the morphology and dynamics of individual ECs within developing vessels. (A) The Tol2(fli1a:H2B-TagBFP-p2A-egfp-F) transgene construct for simultaneously marking endothelial nuclei (with H2B-TagBFP) and EC internal and plasma membranes (with eGFP-farnesyl). (B) Confocal micrograph of a growing trunk ISV/DLAV segment in a 32hpf Tg(kdrl:mRFP-F)^y286 embryo injected with a Tol2(fli1a:H2B-TagBFP-p2A-egfp-F) transgene, showing blue, green and red fluorescent channels and all three merged. This segment contains two individual transgene-labeled ECs (as indicated by the presence of a single H2B-TagBFP nucleus in each). eGFP-F fluorescence is concentrated on the cell surface as well as on internal membranes, although not readily evident in this low-magnification reconstructed image. Scale bar: 20µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development