|ZFIN ID: ZDB-FIG-150413-2|
The p162 mutant phenotype is caused by a nonsense mutation in the foxc1a gene. (A,B) The G to A nucleotide mutation converts tryptophan at position 118 into a premature stop codon. (C) Conceptual translation of the foxc1ap162 predicts a 117 amino acid protein truncated within the forkhead (FH) domain. (D) Injection of wild-type foxc1a mRNA restores axon guidance in foxc1a mutants. (E) Quantification of the mRNA rescue. Numbers on top of individual bars indicate the number of hemisegments and embryos (brackets) analyzed. For all genotypes, only the first six anterior segments were analyzed. Scale bar: 10µm in D.
|GFP||foxc1ap162/p162; ml2Tg||standard conditions||Prim-5||IFL|
|foxc1ap162/p162; ml2Tg||standard conditions||Prim-5||primary motor neuron axon guidance process quality, abnormal|
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