FIGURE

Fig. 5

ID

ZDB-FIG-150316-7

Publication

Maier et al., 2014 - RA and FGF Signalling Are Required in the Zebrafish Otic Vesicle to Pattern and Maintain Ventral Otic Identities

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Fig. 5

Heatshock of Tg(hsp70:dnRAR) embryos leads to an expansion of otic otx1b and a loss of neurod1 expression.

Tg(hsp70:dnRAR) embryos were heat shocked at 18S and sorted into non-green (non-transgenic siblings), green and very green embryos. (A–F) Non-transgenic sibling embryos show normal pattering of the OV assessed by the exp`ression of the non-neural markers tbx1 and otx1b (A,B), the neuronal marker neurod1 (C), and the sensory markers sox2, atoh1a and tecta (D–F) (at least 50 embryos per marker). (G–R) Embryos with low (G–L) or very low (M–R) levels of RA signalling show only a slight change in tbx1 expression (n = 46; G,M), but expression of otx1b is expanded anteroventrally (n = 109; H,N) and expression of neurod1 is reduced (n = 60; I,O). Expression of sox2 (n = 106), atoh1a (n = 150) and tecta (n = 98) is reduced posteriorly (J–L,P–R) and anteriorly (P–R). All panels are lateral views with anterior to the left, apart from the panels depicting tecta (dorsal views; anterior to the left, lateral up). Scale bar: 50 µm.

Expression Data

Genes:	atoh1a neurod1 otx1 sox2 tbx1 tecta
Fish:	pd18Tg
Condition:	heat shock
Anatomical Terms:	anterior macula otic vesicle otic vesicle neuroblast posterior macula
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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