ZFIN ID: ZDB-FIG-141114-6
Hao et al., 2013 - Identification of Estrogen Target Genes during Zebrafish Embryonic Development through Transcriptomic Analysis. PLoS One   8(11):e79020 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Protruding-mouth to Adult

Fig. 6

Validation of co-regulated differentially expressed genes in embryos and adult males using RT-qPCR.

(A) Relative mRNA expression of the up-regulated genes vtg1, vtg3, esr1 , cyp19a1b and f13a1a in adult males upon E2 treatment. (B) Relative mRNA expression of the genes eif4e1b, cyp11a1, dazl, zp3, cpn1, sult1st3 and f2 in 3 dpf and 4 dpf larvae as well as in adult males upon E2 treatment. **P<0.01; unpaired Student’s t-test compared to the controls. Abbreviations eif4e1b: eukaryotic translation initiation factor 4e 1b, cyp11a1 (cytochrome P450, subfamily XIA, polypeptide 1), dazl: deleted in azoospermia-like, zp3: zona pellucida glycoprotein 3, cpn1(carboxypeptidase N, polypeptide 1), sult1st3: sulfotransferase family 1, cytosolic sulfotransferase 3.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cpn1 WT chemical treatment: 17beta-estradiol Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Adult male organism RTPCR
cyp11a1.1 WT chemical treatment: 17beta-estradiol Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Adult male organism RTPCR
cyp19a1b WT chemical treatment: 17beta-estradiol Adult male organism RTPCR
dazl WT chemical treatment: 17beta-estradiol Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Adult male organism RTPCR
eif4e1b WT chemical treatment: 17beta-estradiol Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Adult male organism RTPCR
esr1 WT chemical treatment: 17beta-estradiol Adult male organism RTPCR
f2 WT chemical treatment: 17beta-estradiol Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Adult male organism RTPCR
f13a1a.1 WT chemical treatment: 17beta-estradiol Adult male organism RTPCR
sult1st3 WT chemical treatment: 17beta-estradiol Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Adult male organism RTPCR
vtg1 WT chemical treatment: 17beta-estradiol Adult male organism RTPCR
vtg3 WT chemical treatment: 17beta-estradiol Adult male organism RTPCR
zp3 WT chemical treatment: 17beta-estradiol Protruding-mouth whole organism RTPCR
Day 4 whole organism RTPCR
Adult male organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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