Validation of co-regulated differentially expressed genes in embryos and adult males using RT-qPCR.
(A) Relative mRNA expression of the up-regulated genes vtg1, vtg3, esr1 , cyp19a1b and f13a1a in adult males upon E2 treatment. (B) Relative mRNA expression of the genes eif4e1b, cyp11a1, dazl, zp3, cpn1, sult1st3 and f2 in 3 dpf and 4 dpf larvae as well as in adult males upon E2 treatment. **P<0.01; unpaired Student’s t-test compared to the controls. Abbreviations eif4e1b: eukaryotic translation initiation factor 4e 1b, cyp11a1 (cytochrome P450, subfamily XIA, polypeptide 1), dazl: deleted in azoospermia-like, zp3: zona pellucida glycoprotein 3, cpn1(carboxypeptidase N, polypeptide 1), sult1st3: sulfotransferase family 1, cytosolic sulfotransferase 3.